Proteintech, 66824-1-Ig, COL6A1 Monoclonal antibody

Size: 20ul / 150ul The COL6A1 (66824-1-Ig) by Proteintech is a Monoclonal antibody targeting COL6A1 in WB, IHC, IF-P, ELISA applications with reactivity to human samples 66824-1-Ig targets COL6A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human skeletal muscle tissue, human heart tissue Positive IHC detected in: human liver cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11809 Product name: Recombinant human COL6A1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 770-1028 aa of BC052575 Sequence: NVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG Predict reactive species Full Name: collagen, type VI, alpha 1 Calculated Molecular Weight: 1028 aa, 109 kDa Observed Molecular Weight: 140-150 kDa GenBank Accession Number: BC052575 Gene Symbol: COL6A1 Gene ID (NCBI): 1291 RRID: AB_2882167 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P12109 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.