Proteintech, 66843-1-Ig, CaMKII alpha Monoclonal antibody

Size: 20ul / 150ul The CaMKII alpha (66843-1-Ig) by Proteintech is a Monoclonal antibody targeting CaMKII alpha in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66843-1-Ig targets CaMKII alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: pig brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat rain tissue Positive IHC detected in: mouse brain tissue, human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It has 10 potential isoforms with a molecular mass range from 21 to 63 kDa. This antibody can recognize all the members of the CAMK2 family. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4736 Product name: Recombinant human CAMK2A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-271 aa of BC040457 Sequence: MATITCTRFTEEYQLFEELGKGAFSVVRRCVKVLAGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGHHYLIFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKLKGAAVKLADFGLAIEVEGEQQAWFGFAGTPGYLSPEVLRKDPYGKPVDLWACGVILYILLVGYPPFWDEDQHRLYKQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPSKRITAAEALKHPWI Predict reactive species Full Name: calcium/calmodulin-dependent protein kinase II alpha Calculated Molecular Weight: 478 aa, 54 kDa Observed Molecular Weight: 45-60 kDa GenBank Accession Number: BC040457 Gene Symbol: CaMKII alpha Gene ID (NCBI): 815 RRID: AB_2882184 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UQM7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.