Proteintech, 66854-1-Ig, Phospho-RIPK1 (Ser161) Monoclonal antibody

Size: 20ul / 100ul The Phospho-RIPK1 (Ser161) (66854-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-RIPK1 (Ser161) in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 66854-1-Ig targets Phospho-RIPK1 (Ser161) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, Calyculin A treated HeLa cells, Raji cells, THP-1 cells Positive IHC detected in: human small intestine tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells, Calyculin A treated HeLa cells Positive FC (Intra) detected in: Calyculin A treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:2000- 1:20000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: receptor (TNFRSF)-interacting serine-threonine kinase 1 Calculated Molecular Weight: 76 kDa Observed Molecular Weight: 74 kDa, 45 kDa GenBank Accession Number: NM_003804 Gene Symbol: RIPK1 Gene ID (NCBI): 8737 RRID: AB_2882194 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13546 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.