Proteintech, 66893-1-Ig, TERF2 Monoclonal antibody

Size: 20ul / 150ul The TERF2 (66893-1-Ig) by Proteintech is a Monoclonal antibody targeting TERF2 in WB, ELISA applications with reactivity to human, mouse, rat samples 66893-1-Ig targets TERF2 in WB, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, MCF-7 cells, HT-29 cells, K-562 cells, HEK-293 cells, Daudi cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28513 Product name: Recombinant human TERF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-251 aa of BC024890 Sequence: MAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTVRLGPSPITMVCP Predict reactive species Full Name: telomeric repeat binding factor 2 Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 60-65.32-35 kDa GenBank Accession Number: BC024890 Gene Symbol: TERF2 Gene ID (NCBI): 7014 RRID: AB_2882222 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15554 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.