Proteintech, 66902-3-Ig, AIM2 Monoclonal antibody

Size: 20ul / 150ul The AIM2 (66902-3-Ig) by Proteintech is a Monoclonal antibody targeting AIM2 in WB, ELISA applications with reactivity to human samples 66902-3-Ig targets AIM2 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Daudi cells, Raji cells, Karpas-299 cells, U266 cells, MJ cells, HuT 78 cells, U-87 MG cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949] Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14607 Product name: Recombinant human AIM2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-343 aa of BC010940 Sequence: MESKYKEILLLTGLDNITDEELDRFKFFLSDEFNIATGKLHTANRIQVATLMIQNAGAVSAVMKTIRIFQKLNYMLLAKRLQEEKEKVDKQYKSVTKPKPLSQAEMSPAASAAIRNDVAKQRAAPKVSPHVKPEQKQMVAQQESIREGFQKRCLPVMVLKAKKPFTFETQEGKQEMFHATVATEKEFFFVKVFNTLLKDKFIPKRIIIIARYYRHSGFLEVNSASRVLDAESDQKVNVPLNIIRKAGETPKINTLQTQPLGTIVNGLFVVQKVTEKKKNILFDLSDNTGKMEVLGVRNEDTMKCKEGDKVRLTFFTLSKNGEKLQLTSGVHSTIKVIKAKKKK Predict reactive species Full Name: absent in melanoma 2 Calculated Molecular Weight: 343 aa, 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC010940 Gene Symbol: AIM2 Gene ID (NCBI): 9447 RRID: AB_3670376 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14862 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.