Proteintech, 66920-1-Ig, NFKB2 Monoclonal antibody

Size: 20ul / 150ul The NFKB2 (66920-1-Ig) by Proteintech is a Monoclonal antibody targeting NFKB2 in WB, IHC, ELISA applications with reactivity to human samples 66920-1-Ig targets NFKB2 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, HAP1 cells, WB result of NFKB2 antibody (66920-1-Ig; 1:1000; room temperature for 1.5 hours) with wild-type and NFKB2 knockout HAP1 cells., Jurkat cells, K-562 cells, Daudi cells, THP-1 cells Positive IHC detected in: human liver cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28543 Product name: Recombinant human NFKB2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 600-899 aa of BC002844 Sequence: GLYPVHLAVRARSPECLDLLVDSGAEVEATERQGGRTALHLATEMEELGLVTHLVTKLRANVNARTFAGNTPLHLAAGLGYPTLTRLLLKAGADIHAENEEPLCPLPSPPTSDSDSDSEGPEKDTRSSFRGHTPLDLTCSTKVKTLLLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTEVKEDSAYGSQSVEQEAEKLGPPPEPPGGLCHGHPQPQVH Predict reactive species Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC002844 Gene Symbol: NFKB2 Gene ID (NCBI): 4791 ENSEMBL Gene ID: ENSG00000077150 RRID: AB_2882247 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q00653 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.