Proteintech, 66922-2-Ig, Integrin beta 4 Monoclonal antibody

Size: 20ul / 150ul The Integrin beta 4 (66922-2-Ig) by Proteintech is a Monoclonal antibody targeting Integrin beta 4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66922-2-Ig targets Integrin beta 4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, BxPC-3 cells, SW 1990 cells, SKOV-3 cells, MCF-7 cells, Calu-3 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17395 Product name: Recombinant human Integrin beta-4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 27-354 aa of BC126411 Sequence: ANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSN Predict reactive species Full Name: integrin, beta 4 Calculated Molecular Weight: 1752 aa, 195 kDa Observed Molecular Weight: 200-210 kDa GenBank Accession Number: BC126411 Gene Symbol: Integrin beta 4 Gene ID (NCBI): 3691 RRID: AB_2882249 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P16144 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.