Proteintech, 66924-1-Ig, NFATC3 Monoclonal antibody

Size: 20ul / 150ul The NFATC3 (66924-1-Ig) by Proteintech is a Monoclonal antibody targeting NFATC3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66924-1-Ig targets NFATC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, Daudi cells Positive IHC detected in: human thymus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SKOV-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835) Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Affinity: K D =1.16 x 10 -10 M K Off =5.67 x 10 -6 M K On =4.90 x 10 4 M Immunogen: CatNo: Ag27306 Product name: Recombinant human NFATC3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-205 aa of BC001050 Sequence: MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTL Predict reactive species Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3 Calculated Molecular Weight: 116 kDa Observed Molecular Weight: 160 kDa GenBank Accession Number: BC001050 Gene Symbol: NFATC3 Gene ID (NCBI): 4775 RRID: AB_2882251 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q12968 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.