Proteintech, 66934-1-Ig, PHEX Monoclonal antibody

Size: 20ul / 150ul The PHEX (66934-1-Ig) by Proteintech is a Monoclonal antibody targeting PHEX in WB, IHC, ELISA applications with reactivity to human, rat, pig samples 66934-1-Ig targets PHEX in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples. Tested Applications Positive WB detected in: HEK-293 cells, rat lung tissue, pig lung tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349). Specification Tested Reactivity: human, rat, pig Cited Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20290 Product name: Recombinant human PHEX protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 41-391 aa of BC105059 Sequence: SQGLLSLQAKQEYCLKPECIEAAAAILSKVNLSVDPCDNFFRFACDGWISNNPIPEDMPSYGVYPWLRHNVDLKLKELLEKSISRRRDTEAIQKAKILYSSCMNEKAIEKADAKPLLHILRHSPFRWPVLESNIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSPDDKASNEHILKLDQATLSLAVREDYLDNSTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIKIAEIMIPHENRTSEAMYNKMNISELSAMIPQFDWLGYIKKVIDTRLYPHLKDISPSENVVVRVPQYFKDLFRILGSERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSR Predict reactive species Full Name: phosphate regulating endopeptidase homolog, X-linked Calculated Molecular Weight: 749 aa, 86 kDa Observed Molecular Weight: 76-80 kDa, 95-100 kDa GenBank Accession Number: BC105059 Gene Symbol: PHEX Gene ID (NCBI): 5251 RRID: AB_2882259 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P78562 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.