Proteintech, 66937-1-Ig, UBR5 Monoclonal antibody

Size: 20ul / 150ul The UBR5 (66937-1-Ig) by Proteintech is a Monoclonal antibody targeting UBR5 in WB, IHC, ELISA applications with reactivity to Human, mouse, rat samples 66937-1-Ig targets UBR5 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells, PC-12 cells, HEK-293 cells, Jurkat cells, Neuro-2a cells Positive IHC detected in: mouse testis tissue, mouse brain tissue, rat testis tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse, ae. aegypti Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18753 Product name: Recombinant human UBR5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 2450-2799 aa of BC137234 Sequence: IAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV Predict reactive species Full Name: ubiquitin protein ligase E3 component n-recognin 5 Calculated Molecular Weight: 2799 aa, 309 kDa Observed Molecular Weight: 309 kDa GenBank Accession Number: BC137234 Gene Symbol: UBR5 Gene ID (NCBI): 51366 RRID: AB_2882261 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O95071 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.