Proteintech, 66945-1-Ig, Protein A Monoclonal antibody

Size: 20ul / 150ul The Protein A (66945-1-Ig) by Proteintech is a Monoclonal antibody targeting Protein A in WB, ELISA applications with reactivity to staphylococcus aureus samples 66945-1-Ig targets Protein A in WB, IP, ELISA applications and shows reactivity with staphylococcus aureus samples. Tested Applications Positive WB detected in: recombinant protein Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing. Specification Tested Reactivity: staphylococcus aureus Cited Reactivity: human, mouse, yeast Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Protein A GenBank Accession Number: EU695225 RRID: AB_2882269 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.