Proteintech, 66963-1-Ig, NFATC1 Monoclonal antibody

Size: 20ul / 150ul The NFATC1 (66963-1-Ig) by Proteintech is a Monoclonal antibody targeting NFATC1 in WB, IHC, ELISA applications with reactivity to human samples 66963-1-Ig targets NFATC1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, U2OS cells, Daudi cells, Romas cells, Raji cells, A549 cells, MOLT-4 cells, Ramos cells, Karpas-422 cells Positive IHC detected in: human urothelial carcinoma tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13014 Product name: Recombinant human NFATC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-351 aa of BC104753 Sequence: MPSTSFPVPSKFPLGPAAAVFGRGETLGPAPRAGGTMKSAEEEHYGYASSNVSPALPLPTAHSTLPAPCHNLQTSTPGIIPPADHPSGYGAALDGGPAGYFLSSGHTRPDGAPALESPRIEITSCLGLYHNNNQFFHDVEVEDVLPSSKRSPSTATLSLPSLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGFPRGLGACTLLGSPRHSPSTSPRASVTEESWLGARSSRPASPCNKRKYSLNGRQPPYSPHHSPTPSPHGSPRVSVTDDSWLGNTTQYTSSAIVAAINALTTDSSLDLGDGVPVKSRKTTLEQPPSVALKVE Predict reactive species Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 Calculated Molecular Weight: 716aa,78 kDa; 943aa,101 kDa Observed Molecular Weight: 101 kDa GenBank Accession Number: BC104753 Gene Symbol: NFATC1 Gene ID (NCBI): 4772 RRID: AB_2882286 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O95644 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.