Proteintech, 66976-1-Ig, FOX2/RBM9 Monoclonal antibody

Size: 20ul / 150ul The FOX2/RBM9 (66976-1-Ig) by Proteintech is a Monoclonal antibody targeting FOX2/RBM9 in WB, IHC, ELISA applications with reactivity to Human, Mouse samples 66976-1-Ig targets FOX2/RBM9 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: HeLa cells, HT-29 cells, NCCIT cells, human placenta tissue, fetal human brain tissue, HEK-293 cells, HepG2 cells, L02 cells Positive IHC detected in: mouse brain tissue, human ovary tumor tissue, human lung cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540) Specification Tested Reactivity: Human, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28336 Product name: Recombinant human RBM9 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 6-358 aa of BC013115 Sequence: MVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLIIPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAVAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY Predict reactive species Full Name: RNA binding motif protein 9 Calculated Molecular Weight: 390 aa, 41 kDa Observed Molecular Weight: 48 kDa, 50 and 60 kDa GenBank Accession Number: BC013115 Gene Symbol: RBM9 Gene ID (NCBI): 23543 RRID: AB_2882296 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O43251 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.