Proteintech, 67002-1-Ig, fetuin-B Monoclonal antibody

Size: 20ul / 150ul The fetuin-B (67002-1-Ig) by Proteintech is a Monoclonal antibody targeting fetuin-B in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, rabbit samples 67002-1-Ig targets fetuin-B in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rabbit samples. Tested Applications Positive WB detected in: human plasma, human blood, rabbit serum Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137) Specification Tested Reactivity: human, rabbit Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12463 Product name: Recombinant human fetuin-B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 23-382 aa of BC074734 Sequence: QLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYKNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP Predict reactive species Full Name: fetuin B Calculated Molecular Weight: 382 aa, 42 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC074734 Gene Symbol: Fetuin B Gene ID (NCBI): 26998 RRID: AB_2882319 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UGM5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.