Proteintech, 67017-1-Ig, LGMN Monoclonal antibody

Size: 20ul / 150ul LGMN Monoclonal Antibody for WB, IHC, IF-P, ELISA 67017-1-Ig targets LGMN in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples. Tested Applications Positive WB detected in: SK-BR-3 cells, HEK-293 cells, NCCIT cells, pig kidney tissue, HeLa cells, MCF-7 cells, LNCaP cells, human placenta tissue, JAR cells, Jurkat cells, ATDC-5 cells Positive IHC detected in: human kidney tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human kidney tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471). Specification Tested Reactivity: Human, pig, mouse Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7218 Product name: Recombinant human LGMN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 82-433 aa of BC003061 Sequence: TPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY Predict reactive species Full Name: legumain Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC003061 Gene Symbol: LGMN Gene ID (NCBI): 5641 RRID: AB_2882333 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q99538 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.