Proteintech, 67022-1-Ig, TIMELESS Monoclonal antibody

Size: 20ul / 150ul The TIMELESS (67022-1-Ig) by Proteintech is a Monoclonal antibody targeting TIMELESS in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 67022-1-Ig targets TIMELESS in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, U2OS cells, A549 cells, JAR cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells Positive FC (Intra) detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, canine Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6283 Product name: Recombinant human TIMELESS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 869-1208 aa of BC050557 Sequence: QMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSILLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD Predict reactive species Full Name: timeless homolog (Drosophila) Calculated Molecular Weight: 139 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC050557 Gene Symbol: TIMELESS Gene ID (NCBI): 8914 RRID: AB_2882337 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UNS1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.