Proteintech, 67027-1-Ig, PRC1 Monoclonal antibody

Size: 20ul / 150ul The PRC1 (67027-1-Ig) by Proteintech is a Monoclonal antibody targeting PRC1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67027-1-Ig targets PRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, HepG2 cells, HSC-T6 cells, 4T1 cells Positive IHC detected in: human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5907 Product name: Recombinant human PRC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 154-446 aa of BC003138 Sequence: NQFRQHVTTLRETKASRREEFVSIKRQIILCMEELDHTPDTSFERDVVCEDEDAFCLSLENIATLQKLLRQLEMQKSQNEAVCEGLRTQIRELWDRLQIPEEEREAVATIMSGSKAKVRKALQLEVDRLEELKMQNMKKVIEAIRVELVQYWDQCFYSQEQRQAFAPFCAEDYTESLLQLHDAEIVRLKNYYEVHKELFEGVQKWEETWRLFLEFERKASDPNRFTNRGGNLLKEEKQRAKLQKMLPKLEEELKARIELWEQEHSKAFMVNGQKFMEYVAEQWEMHRLEKERA Predict reactive species Full Name: protein regulator of cytokinesis 1 Calculated Molecular Weight: 72 kDa Observed Molecular Weight: 66-72 kDa GenBank Accession Number: BC003138 Gene Symbol: PRC1 Gene ID (NCBI): 9055 RRID: AB_2882342 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O43663 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.