Proteintech, 67095-1-Ig, EIF3E Monoclonal antibody

Size: 20ul / 150ul The EIF3E (67095-1-Ig) by Proteintech is a Monoclonal antibody targeting EIF3E in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 67095-1-Ig targets EIF3E in WB, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells Positive IP detected in: HeLa cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28523 Product name: Recombinant human EIF3E protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 146-445 aa of BC000734 Sequence: GAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY Predict reactive species Full Name: eukaryotic translation initiation factor 3, subunit E Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48-52 kDa GenBank Accession Number: BC000734 Gene Symbol: EIF3E Gene ID (NCBI): 3646 RRID: AB_2882400 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P60228 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.