Proteintech, 67112-1-Ig, HOXA7 Monoclonal antibody

Size: 20ul / 150ul The HOXA7 (67112-1-Ig) by Proteintech is a Monoclonal antibody targeting HOXA7 in WB, IF/ICC, ELISA applications with reactivity to human samples 67112-1-Ig targets HOXA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, HCT 116 cells, HepG2 cells, HL-60 cells, HT-29 cells, K-562 cells, SMMC-7721 cells, THP-1 cells, ACHN cells, SiHa cells, NCI-H1299 cells, HEK-293 cells, RKO cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information HOX genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene Hoxa-1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor, which has been shown to play important roles in cell differentiation and embryogenesis. Hoxa-1 is also expressed in cancers, such as mammary tumors, though it is not expressed in normal gland or in precancerous mammary tissues. At embryonic stages, Hoxa-2 is expressed in the mesenchyme and epithelial cells of palate, however its expression is restricted to the tips of the growing palatal shelves. Hoxa-2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, Hoxa-2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. Hoxa-7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. Hoxa-7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag19025 Product name: Recombinant human HOXA7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-110 aa of BC148692 Sequence: MSSSYYVNALFSKYTAGASLFQNAEPTSCSFAPNSQRSGYGAGAGAFASTVPGLYNVNSPLYQSPFASGYGLGADAYGNLPCASYDQNIPGLCSDLAKGACDKTDEGALH Predict reactive species Full Name: homeobox A7 Calculated Molecular Weight: 230 aa, 25 kDa Observed Molecular Weight: 20 kDa GenBank Accession Number: BC148692 Gene Symbol: HOXA7 Gene ID (NCBI): 3204 RRID: AB_2882416 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P31268 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.