Product Description
Size: 20ul / 150ul
The USF1 (67114-1-Ig) by Proteintech is a Monoclonal antibody targeting USF1 in WB, ELISA applications with reactivity to Human, mouse, rat samples
67114-1-Ig targets USF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications
Positive WB detected in: HeLa cells, HEK-293 cells, HT-29 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Jurkat cells, PC-12 cells, 4T1 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Background Information
Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa.
USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa
Specification
Tested Reactivity: Human, mouse, rat
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag17785 Product name: Recombinant human USF1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-310 aa of BC035505 Sequence: MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN Predict reactive species
Full Name: upstream transcription factor 1
Calculated Molecular Weight: 34 kDa
Observed Molecular Weight: 38-43 kDa
GenBank Accession Number: BC035505
Gene Symbol: USF1
Gene ID (NCBI): 7391
RRID: AB_2882418
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein G purification
UNIPROT ID: P22415
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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