Proteintech, 67144-1-Ig, URG4 Monoclonal antibody

Size: 20ul / 150ul The URG4 (67144-1-Ig) by Proteintech is a Monoclonal antibody targeting URG4 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples 67144-1-Ig targets URG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: SGC-7901 cells, MKN-45 cells, HepG2 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, human nasopharyngeal carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunohistochemistry (IHC): IHC : 1:100-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence. Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28635 Product name: Recombinant human URG4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-358 aa of BC018426 Sequence: MASPGHSDLGEVAPEIKASERRTAVAIADLEWREMEGDDCEFRYGDGTNEAQDNDFPTVERSRLQEMLSLLGLETYQVQKLSLQDSLQISFDSMKNWAPQVPKDLPWNFLRKLQALNADARNTTMVLDVLPDARPVEKESQMEEEIIYWDPADDLAADIYSFSELPTPDTPVNPLDLLCALLLSSDSFLQQEIALKMALCQFALPLVLPDSENHYHTFLLWAMRGIVRTWWSQPPRGMGSFREDSVVLSRAPAFAFVRMDVSSNSKSQLLNAVLSPGHRQWDCFWHRDLNLGTNAREISDGLVEISWFFPSGREDLDIFPEPVAFLNLRGDIGSHWLQFKLLTEISSAVFILTDNISK Predict reactive species Full Name: up-regulated gene 4 Calculated Molecular Weight: 922 aa, 104 kDa Observed Molecular Weight: 104 kDa GenBank Accession Number: BC018426 Gene Symbol: URG4 Gene ID (NCBI): 55665 RRID: AB_2882443 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8TCY9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.