Proteintech, 67145-1-Ig, CDC25B Monoclonal antibody

Size: 20ul / 150ul The CDC25B (67145-1-Ig) by Proteintech is a Monoclonal antibody targeting CDC25B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 67145-1-Ig targets CDC25B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Sp2/0 cells, Jurkat cells, U-937 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27193 Product name: Recombinant human CDC25B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 169-398 aa of BC006395 Sequence: SQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSK Predict reactive species Full Name: cell division cycle 25 homolog B (S. pombe) Calculated Molecular Weight: 65 kDa Observed Molecular Weight: 69 kDa GenBank Accession Number: BC006395 Gene Symbol: CDC25B Gene ID (NCBI): 994 RRID: AB_2882444 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P30305 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.