Proteintech, 67157-1-Ig, TMPO/LAP2 Monoclonal antibody

Size: 20ul / 150ul The TMPO/LAP2 (67157-1-Ig) by Proteintech is a Monoclonal antibody targeting TMPO/LAP2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67157-1-Ig targets TMPO/LAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, SH-SY5Y cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, K-562 cells Positive IHC detected in: rat brain tissue, human liver cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6415 Product name: Recombinant human TMPO protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-362 aa of BC053675 Sequence: MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISAS Predict reactive species Full Name: thymopoietin Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 51 kDa, 39 kDa GenBank Accession Number: BC053675 Gene Symbol: TMPO/LAP2 Gene ID (NCBI): 7112 RRID: AB_2882454 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P42167 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.