Proteintech, 67163-1-Ig, HIP1 Monoclonal antibody

Size: 20ul / 150ul The HIP1 (67163-1-Ig) by Proteintech is a Monoclonal antibody targeting HIP1 in WB, IHC, IF-P, ELISA applications with reactivity to Human, mouse, pig samples 67163-1-Ig targets HIP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, pig samples. Tested Applications Positive WB detected in: HeLa cells, pig brain tissue, mouse brain tissue, A549 cells, PC-3 cells Positive IHC detected in: human colon cancer tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis. Specification Tested Reactivity: Human, mouse, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17982 Product name: Recombinant human HIP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 435-764 aa of BC110545 Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG Predict reactive species Full Name: huntingtin interacting protein 1 Calculated Molecular Weight: 1037 aa, 116 kDa Observed Molecular Weight: 110-120 kDa GenBank Accession Number: BC110545 Gene Symbol: HIP1 Gene ID (NCBI): 3092 RRID: AB_2882459 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O00291 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.