Proteintech, 67208-1-Ig, IFIT3 Monoclonal antibody

Size: 20ul / 150ul The IFIT3 (67208-1-Ig) by Proteintech is a Monoclonal antibody targeting IFIT3 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human samples 67208-1-Ig targets IFIT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, SGC-7901 cells, Caco-2 cells Positive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins. Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7518 Product name: Recombinant human IFIT3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 141-490 aa of BC004977 Sequence: EEGWTQLKCGRNERAKVCFEKALEEKPNNPEFSSGLAIAMYHLDNHPEKQFSTDVLKQAIELSPDNQYVKVLLGLKLQKMNKEAEGEQFVEEALEKSPCQTDVLRSAAKFYRRKGDLDKAIELFQRVLESTPNNGYLYHQIGCCYKAKVRQMQNTGESEASGNKEMIEALKQYAMDYSNKALEKGLNPLNAYSDLAEFLETECYQTPFNKEVPDAEKQQSHQRYCNLQKYNGKSEDTAVQHGLEGLSISKKSTDKEEIKDQPQNVSENLLPQNAPNYWYLQGLIHKQNGDLLQAAKCYEKELGRLLRDAPSGIGSIFLSASELEDGSEEMGQGAVSSSPRELLSNSEQLN Predict reactive species Full Name: IFIT3 Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC004977 Gene Symbol: IFIT3 Gene ID (NCBI): 3437 RRID: AB_2882501 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14879 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.