Proteintech, 67222-1-Ig, XRCC3 Monoclonal antibody

Size: 20ul / 150ul The XRCC3 (67222-1-Ig) by Proteintech is a Monoclonal antibody targeting XRCC3 in WB, IHC, IF-P, IP, ELISA applications with reactivity to Human, Mouse samples 67222-1-Ig targets XRCC3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: NCI-H1299 cells, HT-29 cells, Caco-2 cells, HT-1080 cells, HeLa cells, MCF-7 cells, COLO 320 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: mouse testis tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Specification Tested Reactivity: Human, Mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13317 Product name: Recombinant human XRCC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC011725 Sequence: MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGAMLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH Predict reactive species Full Name: X-ray repair complementing defective repair in Chinese hamster cells 3 Calculated Molecular Weight: 38 kDa Observed Molecular Weight: 38 kDa GenBank Accession Number: BC011725 Gene Symbol: XRCC3 Gene ID (NCBI): 7517 RRID: AB_2882513 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43542 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.