Proteintech, 67297-1-Ig, KLF6 Monoclonal antibody

Size: 20ul / 150ul The KLF6 (67297-1-Ig) by Proteintech is a Monoclonal antibody targeting KLF6 in WB, ELISA applications with reactivity to human samples 67297-1-Ig targets KLF6 in WB, ChIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: JAR cells, NCCIT cells, L02 cells, Jurkat cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6859 Product name: Recombinant human KLF6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 6-283 aa of BC000311 Sequence: MCSIFQELQIVHETGYFSALPSLEEYWQQTCLELERYLQSEPCYVSASEIKFDSQEDLWTKIILAREKKEESELKISSSPPEDTLISPSFCYNLETNSLNSDVSSESSDSSEELSPTAKFTSDPIGEVLVSSGKLSSSVTSTPPSSPELSREPSQLWGCVPGELPSPGKVRSGTSGKPGDKGNGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHL Predict reactive species Full Name: Kruppel-like factor 6 Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 32-42 kDa GenBank Accession Number: BC000311 Gene Symbol: KLF6 Gene ID (NCBI): 1316 RRID: AB_2882561 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q99612 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.