Proteintech, 67334-1-Ig, ACSM5 Monoclonal antibody

Size: 20ul / 150ul The ACSM5 (67334-1-Ig) by Proteintech is a Monoclonal antibody targeting ACSM5 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67334-1-Ig targets ACSM5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: 4T1 cells, MDA-MB-231 cells, rat liver tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag10056 Product name: Recombinant human ACSM5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-208 aa of BC016703 Sequence: MRPWLRHLVLQALRNSRAFCGSHGKPAPLPVPQKIVATWEAISLGRQLVPEYFNFAHDVLDVWSRLEEAGHRPPNPAFWWVNGTGAEIKWSFEELGKQSRKAANVLGGACGLQPGDRMMLVLPRLPEWWLVSVACMRTGTVVIPGVTQLTEKDLKYRLQASRAKSIITSDSLAPRVDAISAECPSLQTKLLVSDSSRPGWLNFRELLR Predict reactive species Full Name: acyl-CoA synthetase medium-chain family member 5 Calculated Molecular Weight: 208 aa, 23 kDa Observed Molecular Weight: 60-65 kDa GenBank Accession Number: BC016703 Gene Symbol: ACSM5 Gene ID (NCBI): 54988 RRID: AB_2882592 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q6NUN0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.