Proteintech, 67337-1-Ig, SIRP Alpha/CD172a Monoclonal antibody

Size: 20ul / 150ul The SIRP Alpha/CD172a (67337-1-Ig) by Proteintech is a Monoclonal antibody targeting SIRP Alpha/CD172a in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67337-1-Ig targets SIRP Alpha/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig brain tissue, U-251 cells, SW480 cells, HL-60 cells, THP-1 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue Positive IHC detected in: rat cerebellum tissue, human liver tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6361 Product name: Recombinant human SIRPA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 158-508 aa of BC033092 Sequence: ARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK Predict reactive species Full Name: signal-regulatory protein alpha Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 75-85 kDa GenBank Accession Number: BC033092 Gene Symbol: SIRP Alpha Gene ID (NCBI): 140885 RRID: AB_2882595 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P78324 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.