Proteintech, 67350-1-Ig, MLH1 Monoclonal antibody

Size: 20ul / 150ul The MLH1 (67350-1-Ig) by Proteintech is a Monoclonal antibody targeting MLH1 in WB, ELISA applications with reactivity to Human, rat samples 67350-1-Ig targets MLH1 in WB, ELISA applications and shows reactivity with Human, rat samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, Caco-2 cells, COLO 320 cells, HEK-293 cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1. Specification Tested Reactivity: Human, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27723 Product name: Recombinant human MLH1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 407-756 aa of BC006850 Sequence: QPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC Predict reactive species Full Name: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) Calculated Molecular Weight: 756 aa, 85 kDa Observed Molecular Weight: 85-100 kDa, 40-45 kDa GenBank Accession Number: BC006850 Gene Symbol: MLH1 Gene ID (NCBI): 4292 RRID: AB_2918485 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P40692 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.