Proteintech, 67366-1-Ig, CAPN3 Monoclonal antibody

Size: 20ul / 150ul The CAPN3 (67366-1-Ig) by Proteintech is a Monoclonal antibody targeting CAPN3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 67366-1-Ig targets CAPN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: human skeletal muscle tissue, pig skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13179 Product name: Recombinant human CAPN3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-156 aa of BC004883 Sequence: MEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA Predict reactive species Full Name: calpain 3, (p94) Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 90-105 kDa, 55-60 kDa, 30-35 kDa GenBank Accession Number: BC004883 Gene Symbol: CAPN3 Gene ID (NCBI): 825 RRID: AB_2882618 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P20807 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.