Proteintech, 67375-1-Ig, HNRNPH1 Monoclonal antibody

Size: 20ul / 150ul The HNRNPH1 (67375-1-Ig) by Proteintech is a Monoclonal antibody targeting HNRNPH1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67375-1-Ig targets HNRNPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293T cells, HEK-293 cells, MCF-7 cells, LNCaP cells, U2OS cells, HSC-T6 cells, 4T1 cells, Jurkat cells, NIH/3T3 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6603 Product name: Recombinant human HNRNPH1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 104-449 aa of BC001348 Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA Predict reactive species Full Name: heterogeneous nuclear ribonucleoprotein H1 (H) Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 49-50 kDa GenBank Accession Number: BC001348 Gene Symbol: HNRNPH1 Gene ID (NCBI): 3187 RRID: AB_2882623 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P31943 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.