Proteintech, 67385-1-Ig, UBAC1 Monoclonal antibody

Size: 20ul / 150ul The UBAC1 (67385-1-Ig) by Proteintech is a Monoclonal antibody targeting UBAC1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67385-1-Ig targets UBAC1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat brain tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13958 Product name: Recombinant human UBAC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 64-405 aa of BC004967 Sequence: IHAASERVLSDARTILEENIQDQDVLLLIKKRAPSPLPKMADVSAEEKKKQDQKAPDKEAILRATANLPSYNMDRAAVQTNMRDFQTELRKILVSLIEVAQKLLALNPDAVELFKKANAMLDEDEDERVDEAALRQLTEMGFPENRATKALQLNHMSVPQAMEWLIEHAEDPTIDTPLPGQAPPEAEGATAAASEAAAGASATDEEARDELTEIFKKIRRKREFRADARAVISLMEMGFDEKEVIDALRVNNNQQNAACEWLLGDRKPSPEELDKGIDPDSPLFQAILDNPVVQLGLTNPKTLLAFEDMLENPLNSTQWMNDPETGPVMLQISRIFQTLNRT Predict reactive species Full Name: UBA domain containing 1 Calculated Molecular Weight: 405 aa, 45 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC004967 Gene Symbol: UBAC1 Gene ID (NCBI): 10422 RRID: AB_2882630 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9BSL1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.