Proteintech, 67398-1-Ig, Caspase 4 Monoclonal antibody

Size: 20ul / 150ul The Caspase 4 (67398-1-Ig) by Proteintech is a Monoclonal antibody targeting Caspase 4 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human samples 67398-1-Ig targets Caspase 4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29488 Product name: Recombinant human Caspase 4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-377 aa of BC017839 Sequence: MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN Predict reactive species Full Name: caspase 4, apoptosis-related cysteine peptidase Calculated Molecular Weight: 377 aa, 43 kDa Observed Molecular Weight: 43-48 kDa GenBank Accession Number: BC017839 Gene Symbol: Caspase 4 Gene ID (NCBI): 837 RRID: AB_2882641 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P49662 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.