Proteintech, 67402-1-Ig, AGXT Monoclonal antibody

Size: 20ul / 150ul The AGXT (67402-1-Ig) by Proteintech is a Monoclonal antibody targeting AGXT in WB, IHC, ELISA applications with reactivity to human samples 67402-1-Ig targets AGXT in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, NCI-H1299 cells, A549 cells, L02 cells, HuH-7 cells, SMMC-7721 cells Positive IHC detected in: human liver cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag19188 Product name: Recombinant human AGXT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-392 aa of BC132819 Sequence: MASHKLLVTPPKALLKPLSIPNQLLLGPGPSNLPPRIMAAGGLQMIGSMSKDMYQIMDEIKEGIQYVFQTRNPLTLVISGSGHCALEAALVNVLEPGDSFLVGANGIWGQRAVDIGERIGARVHPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGSQKALNAPPGTSLISFSDKAKKKMYSRKTKPFSFYLDIKWLANFWGCDDQPRMYHHTIPVISLYSLRESLALIAEQGLENSWRQHREAAAYLHGRLQALGLQLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGKVLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL Predict reactive species Full Name: alanine-glyoxylate aminotransferase Calculated Molecular Weight: 392 aa, 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC132819 Gene Symbol: AGXT Gene ID (NCBI): 189 RRID: AB_2882644 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P21549 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.