Proteintech, 67428-1-Ig, eIF4G2/DAP5 Monoclonal antibody

Size: 20ul / 150ul The eIF4G2/DAP5 (67428-1-Ig) by Proteintech is a Monoclonal antibody targeting eIF4G2/DAP5 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67428-1-Ig targets eIF4G2/DAP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, LNCaP cells, K-562 cells Positive IHC detected in: human prostate cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12080 Product name: Recombinant human EIF4G2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 563-907 aa of BC043149 Sequence: EAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD Predict reactive species Full Name: eukaryotic translation initiation factor 4 gamma, 2 Calculated Molecular Weight: 907 aa, 102 kDa Observed Molecular Weight: 97 kDa GenBank Accession Number: BC043149 Gene Symbol: EIF4G2 Gene ID (NCBI): 1982 RRID: AB_2882667 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P78344 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.