Proteintech, 67447-1-Ig, c-MYC Monoclonal antibody

Size: 20ul / 150ul The c-MYC (67447-1-Ig) by Proteintech is a Monoclonal antibody targeting c-MYC in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 67447-1-Ig targets c-MYC in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MDA-MB-231 cells, HT-29 cells, 4T1 cells, PC-3 cells, K-562 cells, HL-60 cells, Raji cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue, mouse brain tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: K-562 cells, NCCIT cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, bacillus subtilis Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17519 Product name: Recombinant human MYC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 16-454 aa of BC000141 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA Predict reactive species Full Name: v-myc myelocytomatosis viral oncogene homolog (avian) Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC000141 Gene Symbol: MYC Gene ID (NCBI): 4609 RRID: AB_2882681 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P01106 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.