Proteintech, 67463-1-Ig, ZNF746 Monoclonal antibody

Size: 20ul / 150ul The ZNF746 (67463-1-Ig) by Proteintech is a Monoclonal antibody targeting ZNF746 in WB, ELISA applications with reactivity to human samples 67463-1-Ig targets ZNF746 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, LNCaP cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN: its accumulation due to the absence of PRKN, followed by up-regulation of PPARGC1A, could lead to the selective loss of dopamine neurons in the substantia nigra(PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag21582 Product name: Recombinant human ZNF746 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 186-531 aa of BC068505 Sequence: MQIKQEGELQLQEQQALGVEAWAAGQPDIGEEPWGLSQLDSGAGDISTDATSGVHSNFSTTIPPTSWQTDLPPHHPSSACSDGTLKLNTAASTEDVKIVIKTEVQEEEVVATPVHPTDLEAHGTLFGPGQATRFFPSPAQEGAWESQGSSFPSQDPVLGLREPARPERDMGELSPAVAQEETPPGDWLFGGVRWGWNFRCKPPVGLNPRTGPEGLPYSSPDNGEAILDPSQAPRPFNEPCKYPGRTKGFGHKPGLKKHPAAPPGGRPFTCATCGKSFQLQVSLSAHQRSCGAPDGSGPGTGGGGSGSGGGGGGSGGGSARDGSALRCGECGRCFTRPAHLIRHRML Predict reactive species Full Name: zinc finger protein 746 Calculated Molecular Weight: 644 aa, 69 kDa Observed Molecular Weight: 85-90 kDa GenBank Accession Number: BC068505 Gene Symbol: ZNF746 Gene ID (NCBI): 155061 RRID: AB_2918491 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q6NUN9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.