Proteintech, 67483-1-Ig, PIP5K1A Monoclonal antibody

Size: 20ul / 150ul The PIP5K1A (67483-1-Ig) by Proteintech is a Monoclonal antibody targeting PIP5K1A in WB, IHC, ELISA applications with reactivity to human samples 67483-1-Ig targets PIP5K1A in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: LNCaP cells, HEK-293 cells, HepG2 cells, L02 cells, Jurkat cells, Hela cell, K-562 cells, MOLT-4 cells, HeLa cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8355 Product name: Recombinant human PIP5K1A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 151-500 aa of BC007833 Sequence: SLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH Predict reactive species Full Name: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha Calculated Molecular Weight: 56 kDa, 63 kDa Observed Molecular Weight: 63 kDa GenBank Accession Number: BC007833 Gene Symbol: PIP5K1A Gene ID (NCBI): 8394 RRID: AB_2882710 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99755 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.