Proteintech, 67501-1-Ig, RAB2A Monoclonal antibody

Size: 20ul / 150ul The RAB2A (67501-1-Ig) by Proteintech is a Monoclonal antibody targeting RAB2A in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 67501-1-Ig targets RAB2A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U2OS cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Positive IF/ICC detected in: U2OS cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7939 Product name: Recombinant human RAB2A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-212 aa of BC008929 Sequence: MAYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTTWLEDARQHSNSNMVIMLIGNKSDLESRREVKKEEGEAFAREHGLIFMETSAKTASNVEEAFINTAKEIYEKIQEGVFDINNEANGIKIGPQHAATNATHAGNQGGQQAGGGCC Predict reactive species Full Name: RAB2A, member RAS oncogene family Calculated Molecular Weight: 24 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC008929 Gene Symbol: RAB2A Gene ID (NCBI): 5862 RRID: AB_2882725 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P61019 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.