Proteintech, 67514-1-Ig, ZEB2 Monoclonal antibody

Size: 20ul / 150ul The ZEB2 (67514-1-Ig) by Proteintech is a Monoclonal antibody targeting ZEB2 in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 67514-1-Ig targets ZEB2 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, HT-1080 cells, NIH/3T3 cells, HSC-T6 cells, 4T1 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 ) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag25477 Product name: Recombinant human ZEB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 402-658 aa of BC127102 Sequence: VLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSV Predict reactive species Full Name: zinc finger E-box binding homeobox 2 Observed Molecular Weight: 160 kDa GenBank Accession Number: BC127102 Gene Symbol: ZEB2 Gene ID (NCBI): 9839 RRID: AB_2882735 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O60315 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.