Proteintech, 67529-1-Ig, PAX6 Monoclonal antibody

Size: 20ul / 150ul The PAX6 (67529-1-Ig) by Proteintech is a Monoclonal antibody targeting PAX6 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 67529-1-Ig targets PAX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A-673 cells, HeLa cells, hTERT-RPE1 cells, JAR cells, K-562 cells, Neuro-2a cells, rat cerebellum tissue, SW480 cells, HT-1376 cells, MG-63 cells, rat retina tissue, mouse retina tissue, rabbit retina tissue Positive IHC detected in: mouse eye tissue, mouse brain tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29977 Product name: Recombinant human PAX6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-330 aa of BC011953 Sequence: MQNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATPEVVSKIAQYKRECPSIFAWEIRDRLLSEGVCTNDNIPSVSSINRVLRNLASEKQQMGADGMYDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQDGCQQQEGGGENTNSISSNGEDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTHYPDVFARERLAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASNTPSHIPISSSFSTSVYQPIPQPTTPVSSFTSGSMLGRTDTALTNTYSALP Predict reactive species Full Name: paired box 6 Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC011953 Gene Symbol: PAX6 Gene ID (NCBI): 5080 RRID: AB_2882748 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P26367 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.