Proteintech, 67540-1-Ig, TCP1 eta Monoclonal antibody

Size: 20ul / 150ul The TCP1 eta (67540-1-Ig) by Proteintech is a Monoclonal antibody targeting TCP1 eta in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 67540-1-Ig targets TCP1 eta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, NIH/3T3 cells, HEK-293 cells, LNCaP cells, Hela cells, HepG cells, Jurkat cells, K-562 cells Positive IHC detected in: mouse kidney tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9502 Product name: Recombinant human CCT7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 240-543 aa of BC019296 Sequence: LNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLEKIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGRCQVFEETQIGGERYNFFTGCPKAKTCTFILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGTWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVDAPTAAGRGRGRGRPH Predict reactive species Full Name: chaperonin containing TCP1, subunit 7 (eta) Calculated Molecular Weight: 543 aa, 59 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC019296 Gene Symbol: CCT7 Gene ID (NCBI): 10574 RRID: AB_2882759 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q99832 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.