Proteintech, 67648-1-Ig, GAD1 Monoclonal antibody

Size: 20ul / 150ul The GAD1 (67648-1-Ig) by Proteintech is a Monoclonal antibody targeting GAD1 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67648-1-Ig targets GAD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: pig brain tissue, rat brain tissue, mouse cerebellum tissue, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue, rat cerebellum tissue, mouse brain tissue Positive IHC detected in: rat cerebellum tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat brain tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag21336 Product name: Recombinant human GAD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-224 aa of BC002815 Sequence: MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMPSDMRECWLLR Predict reactive species Full Name: glutamate decarboxylase 1 (brain, 67kDa) Calculated Molecular Weight: 67 kDa Observed Molecular Weight: 67 kDa, 57 kDa GenBank Accession Number: BC002815 Gene Symbol: GAD1 Gene ID (NCBI): 2571 RRID: AB_2882848 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99259 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.