Proteintech, 67661-1-Ig, ETFA Monoclonal antibody

Size: 20ul / 150ul The ETFA (67661-1-Ig) by Proteintech is a Monoclonal antibody targeting ETFA in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 67661-1-Ig targets ETFA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HCT 116 cells, LNCaP cells, mouse brain tissue, HuH-7 cells, HeLa cells, K-562 cells, pig liver tissue, rat liver tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:8000-1:32000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302) Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30257 Product name: Recombinant human ETFA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-333 aa of BC015526 Sequence: MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK Predict reactive species Full Name: electron-transfer-flavoprotein, alpha polypeptide Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC015526 Gene Symbol: ETFA Gene ID (NCBI): 2108 RRID: AB_2882858 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13804 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.