Proteintech, 67676-1-Ig, PCK2 Monoclonal antibody

Size: 20ul / 150ul The PCK2 (67676-1-Ig) by Proteintech is a Monoclonal antibody targeting PCK2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67676-1-Ig targets PCK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: HuH-7 cells, rat liver tissue, pig liver tissue, rabbit liver tissue, HepG2 cells, LNCaP cells, K562 cells, Neuro-2a cells, HSC-T6 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human lung cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:4000-1:16000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7021 Product name: Recombinant human PCK2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM Predict reactive species Full Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial) Calculated Molecular Weight: 71 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC001454 Gene Symbol: PCK2 Gene ID (NCBI): 5106 RRID: AB_2882871 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q16822 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.