Proteintech, 67723-1-Ig, PSIP1 Monoclonal antibody

Size: 20ul / 150ul The PSIP1 (67723-1-Ig) by Proteintech is a Monoclonal antibody targeting PSIP1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 67723-1-Ig targets PSIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: LNCaP cells, Jurkat cells, U2OS cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators. Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30562 Product name: Recombinant human PSIP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 104-325 aa of NM_021144 Sequence: ASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETE Predict reactive species Full Name: PC4 and SFRS1 interacting protein 1 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: NM_021144 Gene Symbol: PSIP1 Gene ID (NCBI): 11168 RRID: AB_3670379 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75475 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.