Proteintech, 67731-1-Ig, DFNA5/GSDME Monoclonal antibody

Size: 20ul / 150ul The DFNA5/GSDME (67731-1-Ig) by Proteintech is a Monoclonal antibody targeting DFNA5/GSDME in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig samples 67731-1-Ig targets DFNA5/GSDME in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: pig brain tissue, HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue Positive FC (Intra) detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30514 Product name: Recombinant human DFNA5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 111-460 aa of BC019689 Sequence: SQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISL Predict reactive species Full Name: deafness, autosomal dominant 5 Calculated Molecular Weight: 496 aa, 55 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC019689 Gene Symbol: DFNA5 Gene ID (NCBI): 1687 RRID: AB_2882917 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O60443 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.