Proteintech, 67732-1-Ig, CAPN1 Monoclonal antibody

Size: 20ul / 150ul The CAPN1 (67732-1-Ig) by Proteintech is a Monoclonal antibody targeting CAPN1 in WB, IHC, ELISA applications with reactivity to Human samples 67732-1-Ig targets CAPN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, LNCaP cells, T-47D cells, K562 cells Positive IHC detected in: human urothelial carcinoma tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Specification Tested Reactivity: Human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30522 Product name: Recombinant human CAPN1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 413-714 aa of BC008751 Sequence: RESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQANLPDEQVLSEEEIDENFNALFRQLAGEDMEISVKELRTILNRIISKHKDLRTKGFSLESCRSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKLYELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA Predict reactive species Full Name: calpain 1, (mu/I) large subunit Calculated Molecular Weight: 704 aa, 82 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC008751 Gene Symbol: CAPN1 Gene ID (NCBI): 823 RRID: AB_2918501 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07384 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.