Proteintech, 67744-1-Ig, PTPN14 Monoclonal antibody

Size: 20ul / 150ul The PTPN14 (67744-1-Ig) by Proteintech is a Monoclonal antibody targeting PTPN14 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 67744-1-Ig targets PTPN14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U2OS cells, A549 cells, HEK-293 cells, Hela cells, K-562 cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12679 Product name: Recombinant human PTPN14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-351 aa of BC101754 Sequence: MPFGLKLRRTRRYNVLSKNCFVTRIRLLDSNVIECTLSVESTGQECLEAVAQRLELRETHYFGLWFLSKSQQARWVELEKPLKKHLDKFANEPLLFFGVMFYVPNVSWLQQEATRYQYYLQVKKDVLEGRLRCTLDQVIRLAGLAVQADFGDYNQFDSQDFLREYVLFPMDLALEEAVLEELTQKVAQEHKAHSGILPAEAELMYINEVERLDGFGQEIFPVKDNHGNCVHLGIFFMGIFVRNRIGRQAVIYRWNDMGNITHNKSTILVELINKEETALFHTDDIENAKYISRLFATRHKFYKQNKICTEQSNSPPPIRRQPTWSRSSLPRQQPYILPPVHVQCGEHYSET Predict reactive species Full Name: protein tyrosine phosphatase, non-receptor type 14 Calculated Molecular Weight: 1187 aa, 135 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC101754 Gene Symbol: PTPN14 Gene ID (NCBI): 5784 RRID: AB_2918513 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15678 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.